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Abstract: The use of novel tobacco products that do not burn tobacco leaves is becoming increasingly popular worldwide. In terms of respiratory system, smoking damage to the epithelial cells of lung is in urgent need of attention and research. In this study, we produced the combustion cigarettes (CC) condensate from conventional cigarettes and the heated tobacco products (HTP) condensate from heat-not-burn (HNB) tobacco, and determine the nicotine and other ingredients content respectively to assessment the CC and HTP cytotoxicity after acute exposure to cigarette smoke. We further select a toxicological dose for comparison of the difference between the CC and HTP, which the impacts on BEAS-2B and A549 cells at a 70% survival rate was evaluated. Then, biological effects of CC and HTP on BEAS-2B and A549 cells, which including cell viability, apoptosis and cell cycle were investigated. Results showed that the BEAS-2B and A549 cells survival rate was decreased with increasing dose of CC and HTP smoke trap for 24 h. Both CC and HTP could suppress the proliferation of BEAS-2B cells, though the degree of inhibition of cell proliferation in CC group was greater than in HTP group. The CC and HTP traps could promote apoptosis in BEAS-2B and A549 cells. The apoptosis rate of HTP group was less than that of CC group. In addition, CC and HTP traps could partly restrain cell cycle progression in G0/G1 phase. These results demonstrate that CC and HTP can cause cytotoxicity, apoptosis and affect cell cycle progression, which thus may be useful to elucidate the underlying biological effects of novel tobacco products and provide new ideas for the preventing and treating diseases.Abstract: The use of novel tobacco products that do not burn tobacco leaves is becoming increasingly popular worldwide. In terms of respiratory system, smoking damage to the epithelial cells of lung is in urgent need of attention and research. In this study, we produced the combustion cigarettes (CC) condensate from conventional cigarettes and the heated tob...Learn More
Abstract: The injection-dry application technique is a proactive technique to prevent Pine wilt disease. The study selected Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan six districts and counties (units) of the big (ancient) Pine (Pinus massoniana) as the research object, the use of injection of dry application method to clarify the prevention of Pine wilt disease by 2.3% Emamectin Benzoate and 5% abamectin, the use of high performance liquid chromatography determination of the injection of dry application of 180d and High performance liquid chromatography (HPLC) was used to determine the residue of Emamectin Benzoate in Pinus sylvestris after 180d and 360d of dry application. The results showed that Emamectin Benzoate and abamectin injection dry application of six districts and counties (units) of Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan, large (ancient) Pine wilt disease prevention and control effect is significant, the prevention effect of the consecutive 2a have reached more than 99%; In addition, the establishment of the pony pine in the Emamectin Benzoate and abamectin residue detection method, the side branch in the Emamectin Benzoate and abamectin In addition, a method was established to detect the residues of Emamectin Benzoate and abamectin in Pinus sylvestris, and the recoveries of Emamectin Benzoate and abamectin in the lateral branches exceeded 80%, and the analytical methods of Emamectin Benzoate and abamectin met the requirements of residue detection; the highest residue concentrations of Emamectin Benzoate and abamectin in the lateral branches of Pinus sylvestris were 2.93±1.63mg kg-1 and 2.93±0.69mg kg-1, respectively, 180d after injection drying. In the second year, the residue concentrations decreased, and the highest concentrations were more than 1.93±1.4 mg kg-1 and 0.71±0.49 mg kg-1 in the lateral branches of pine trees injected with mevinphos and abamectin, respectively.Abstract: The injection-dry application technique is a proactive technique to prevent Pine wilt disease. The study selected Zhongxian, Banan, Beibei District Jinyun Mountain National Nature Reserve, Wulong, Liangping, Hechuan six districts and counties (units) of the big (ancient) Pine (Pinus massoniana) as the research object, the use of injection of dry ap...Learn More
