巴氏杀菌乳、UHT灭菌乳中乳果糖项目的检测研究
内蒙古蒙牛乳业科尔沁有限责任公司, 检测实验室, 内蒙古通辽 028000
| 摘 要： | 牛奶富含各种营养物质，其中的功能性蛋白，对人体健康有非常多的益处。但是牛奶在上市前都要经过热处理。牛奶热处理的强度越强，功能性蛋白损失越多。乳果糖是牛奶中重要的热敏指标之一，可以指示牛奶的热处理强度。农业部畜牧业司提出对巴氏杀菌乳进行乳果糖检测，现方法由于操作过程复杂、检测耗时长、且需要对酶活力进行换算等因素影响，不能快速满足业务需求，采用乳果糖快检试剂盒，在保证检测结果准确性的前提下，提高了检测效率，同时也缩短了检测时间。本文详细介绍了乳果糖试剂盒（馥申）检测法从实验室材料准备、检测操作关键步骤注意项及对比数据分析等方面，得出了试剂盒法在精准性、检测时间、操作便捷度、检测费用等方面均优于传统检测方法。 |
| 关 键 词： | 乳果糖; 快检试剂盒; 牛乳; 快速; 准确 |
| DOI： | 10.57237/j.wjfse.2024.01.002 |
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收稿日期: 2024-10-09
接受日期: 2024-11-12
在线出版日期: 2024-12-05
Research on the Detection of Lactulose in Pasteurized Milk and UHT Sterilized Milk
Testing Laboratory, Inner Mongolia Mengniu Dairy Horqin Co., Ltd., Tongliao 028000, China
| Abstract: | Milk is rich in various nutrients, including functional proteins, which have many benefits for human health. However, milk must undergo heat treatment before being marketed. The stronger the heat treatment of milk, the greater the loss of functional proteins. Lactulose is one of the important heat sensitive indicators in milk, which can indicate the heat treatment intensity of milk. The Animal Husbandry Department of the Ministry of Agriculture proposed to conduct lactulose detection on pasteurized milk. However, the current method cannot quickly meet business needs due to complex operation, long detection time, and the need to convert enzyme activity. Therefore, a lactulose rapid detection kit is used to improve detection efficiency and shorten detection time while ensuring the accuracy of detection results. This article provides a detailed introduction to the detection method of lactulose test kit (Fushen) from the aspects of laboratory material preparation, key steps and precautions in detection operation, and comparative data analysis. It is concluded that the test kit method is superior to traditional detection methods in terms of accuracy, detection time, ease of operation, and detection cost. |
| Keywords: | Lactulose; Rapid Testing Kit; Milk; Quickly; Accurate |
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