黄芪多糖对脂多糖诱导人甲状腺细胞iNOS-NO的作用研究
1. 锦州医科大学, 附属第一医院老年医学科, 辽宁锦州 121001
2. 锦州医科大学, 药理学教研室, 辽宁锦州 121001
| 摘 要： | 目的：研究黄芪多糖对脂多糖（Lipopolysaccharide，LPS）诱导体外培养人甲状腺细胞诱导性一氧化氮合酶（Inducible nitric oxide synthase，iNOS）作用的影响。方法：采用体外原代细胞培养方法，观察LPS在不同浓度（0、1、10、100μg•ml-1）及同一浓度（100μg•ml-1）不同时间（6、12、18、24h）对人甲状腺细胞生成一氧化氮（Nitric oxide，NO）的影响；L-NAME（iNOS抑制剂）对LPS上述作用的影响；黄芪多糖对LPS上述作用的影响。结果：LPS（0、1、10、100 μg•ml-1）呈剂量依赖性促进甲状腺细胞生成NO；LPS（100 μg•ml-1）（6、12、18、24h）呈时间依赖性促进甲状腺细胞生成NO；L-NAME抑制LPS的上述作用；黄芪多糖抑制LPS 的上述作用。结论：黄芪多糖能抑制LPS诱导甲状腺细胞iNOS合成NO，黄芪多糖可能对感染所致甲状腺相关疾病有治疗作用。 |
| 关 键 词： | 黄芪多糖; 脂多糖; 诱导性一氧化氮合酶; 一氧化氮; 甲状腺 |
| DOI： | 10.57237/j.wjcm.2023.03.001 |
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收稿日期: 2023-05-31
接受日期: 2023-07-14
在线出版日期: 2023-07-24
Effects of Astragalus Polysaccharides on iNOS-NO Induced by Lipopolysaccharide in Human Thyroid Cells
1. The Department of Geriatrics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121001, China
2. The Department of Pharmacology, Jinzhou Medical University, Jinzhou 121001, China
| Abstract: | Objective: To study the effects of Astragalus polysaccharides on the induction of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide (LPS) in human thyroid cells in vitro. Methods: Thyrocytes obtained from paraadenomatous tissue of thyroid adenomas were cultured in a monolayer system. Human thyrocytes were treated with LPS at different concentration (0, 1, 10, 100μg • ml-1) and at the same concentration for different time (6, 12, 18, 24 h). The level of nitric oxide (NO) in the culture medium was observed; Effect of iNOS inhibitor L-NAME on the above-mentioned effects of LPS; Effect of Astragalus polysaccharides on the above-mentioned effects of LPS. Results: (1) LPS (0, 1, 10, 100μg•ml-1) significantly increased the level of NO in dose-dependent manner. (2) LPS (6, 12, 18, 24 h) significantly increased the level of NO in time-dependent manner at the same concentration. (3) L-NAME inhibited the above-mentioned effects of LPS. (4) Astragalus polysaccharides inhibited the above-mentioned effects of LPS. Conclusions: Astragalus polysaccharides inhibited LPS-induced NO synthesis in iNOS in thyroid cells. Astragalus polysaccharides might have therapeutic effects on thyroid-related diseases caused by LPS-mediated infection. |
| Keywords: | Astragalus Polysaccharides; Lipopolysaccharide; Inducible Nitric Oxide Synthase; Nitric Oxide; Thyroid |
基金
| 1. | 辽宁省锦州市指导性科技计划项目 (JZ2023B057) |
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