终末期肝病干细胞与组织工程修复的关键策略
1. 陆军军医大学, 第一附属医院全军肝胆外科研究所肝胆外科, 重庆 400038
2. 山西医科大学, 第一附属特种医学部, 山西太原 030000
3. 北京大学第三附属医院, 骨科, 北京 100191
| 摘 要： | 终末期肝病（ESLD）是各种慢性肝脏疾病的终结点，临床发病和死亡率均高，目前尚无有效根治药物。肝移植（LTx）是ESLD最佳治疗方案，但限于肝源供体束缚了LTx展开。为解决这一瓶颈问题，研究者们努力探索，相继尝试了人工肝、干细胞、生物组织工程肝、异种移植肝等技术以期对ESLD修复替代的治疗作用，尤其成体干细胞干预和采用脱细胞化/再细胞化工程策略构建功能性大体积肝组织近年备受关注，且这项技术已成为当今构建复杂器官的首选。前期我们在中华消化外科杂志上就脱细胞肝支架材料优化工程肝与ESLD替代治疗方面讨论了几点问题。现就ESLD干细胞干预，以及其作为种子细胞在脱细胞肝支架重建肝组织主要结构修复替代等问题研究进展、面临挑战和未来预期进行予以综述，希望领域内人们有所启发，为解决干细胞移植疗效不稳定和肝源短缺问题提供思考。 |
| 关 键 词： | 组织工程; 终末期肝病; 肝移植; 干细胞; 脱细胞支架; 肝内胆管; 肝血窦 |
| DOI： | 10.57237/j.mrf.2023.01.003 |
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收稿日期: 2022-12-09
接受日期: 2023-02-09
在线出版日期: 2023-02-16
Key Issues and Novel Strategies of Stem Cell Intervention and Decellularized Scaffold Liver Tissue Engineer for End-Stage Liver Disease
1. Hepatobiliary Institute, Southwest Hospital, Army Medical University, Chongqing 400038, China
2. Department of Orthopaedics, Pinking University Third Hospital, Beijing 100191, China
| Abstract: | End-stage liver disease (ESLD) is ultimation of all kinds of chronic liver diseases. It is highly morbidity and mortality disease and currently no effective treatment is available. Liver transplantation (LTx) is the only way for the treatment. However, the organ source limitation interferes with clinical application of LTx. For the goal of crossing liver source shortage barrier, researches attempt to explore techniques or methods like stem cells and tissue engineering, and recent years sounds developed rapidly and bright prospects, for example, artificial liver, tissue-engineered liver and xenotransplantation etc., in particular adult stem cells like bone marrow-derived mesenchymal stem cells (BMMSCs) and decellularized liver scaffold for large scale liver tissue engineer seems more eye-catching, and such decellularization / recellularization technology used has become the first strategy to build complex organs. We have previously discussed some points in the |
| Keywords: | Tissue Engineering; End-Stage Liver Disease; Liver Transplantation; Stem Cells; Decellularized Liver Scaffold; Bilinary Epithelial Cells; Hepatic Sinusoid |
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