基于密勒胺的电化学发光检测过氧化氢
华北理工大学, 材料科学与工程学院, 河北唐山 063210
| 摘 要： | 过氧化氢（H2O2）是最重要的活性氧（ROS）之一，与各种病理和生理过程有关。此外，由于癌细胞比正常细胞产生过量的H2O2，H2O2可以用作评估不同细胞中氧化应激能力差异和检测癌细胞的生物标志物。因此，有效和准确地检测H2O2对于生物学研究至关重要。电化学发光检测具有灵敏度高，操作简单的优点。melem作为一种生物环保材料的同时，还具有一定的发光性能，在电化学发光检测领域具有一定的应用价值。本文采用热聚合结合硝酸氧化技术制备了具有良好发光性能的氧化型密勒胺（melem-AT）。以melem-AT为发光体，过硫酸钾为共反应剂，基于H2O2对melem-AT的电化学发光（ECL）协同增强作用，构建了H2O2传感器。在最佳的实验条件下，melem-AT发光强度差值与H2O2浓度呈现良好的线性关系，检测范围为0.125 ~ 35 mol/L，检测限为100 nmol/L。人体血清中H2O2的检测结果表明，ECL传感器在临床检测中具有潜在的应用价值。与其他方法相比，该传感器具有操作简单、成本低、检测速度快等优点，为H2O2的检测提供了一种有前途的方法。 |
| 关 键 词： | 电化学发光; 密勒胺; 过氧化氢 |
| DOI： | 10.57237/j.mater.2024.05.002 |
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收稿日期: 2024-08-17
接受日期: 2024-09-24
在线出版日期: 2024-10-26
Electrochemical Luminescence Detection of Hydrogen Peroxide Based on Melem
College of Materials Science and Engineering, North China University of Science and Technology, Tangshan 063210, China
| Abstract: | Hydrogen peroxide (H2O2) is one of the most important reactive oxygen species (ROS), involved in various pathological and physiological processes. Additionally, since cancer cells produce an excess of H2O2 compared to normal cells, H2O2 can serve as a biomarker for assessing differences in oxidative stress capabilities among different cells and for detecting cancer cells. Therefore, effective and accurate detection of H2O2 is crucial for biological research. Electrochemiluminescence detection offers the advantages of high sensitivity and simple operation. Melem, as an eco-friendly biological material, also possesses certain luminescent properties, making it valuable for application in the field of electrochemiluminescence detection. In this paper, oxidized melamine (melem-AT) with good luminescent property was prepared by thermal polymerization combined with nitric acid oxidation. Based on the synergistic effect of H2O2 on electrochemiluminescence (ECL) of melem-AT, a H2O2 sensor was constructed using melem-AT as luminescent substance and potassium persulfate as co-reaction agent. Under the best experimental conditions, there is a good linear relationship between the luminous intensity of melem-AT and H2O2 concentration. The detection range is 0.125 ~ 35 mol/L with detection limit of 100 nmol/L. The detection results of H2O2 in human serum show that ECL sensor has potential application value in clinical detection. Compared to other methods, this sensor offers advantages such as simple operation, low cost, and fast detection speed, providing a promising approach for the detection of H2O2. |
| Keywords: | Electrochemiluminescence; Melem; Hydrogen Peroxide |
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