小麦蛋白激酶基因TaSnRK2.1介导植株抵御干旱胁迫功能研究
河北农业大学, 农学院, 河北保定 071001
| 摘 要： | SnRK2型蛋白激酶通过介导特定发育和逆境信号传递，在调控植植株生长发育和介导植株抵御非生物逆境胁迫中发挥着重要作用。本研究分析了小麦种属SnRK2蛋白激酶家族基因TaSnRK2.1分子特征、应答干旱表达模式和介导植株抵御干旱胁迫的生物学功能。结果表明，TaSnRK2.1与波兰小麦和大麦等种属中SnRK2激酶基因序列高度同源，编码蛋白含有Ser/Thr激酶具有的保守结构域（aa 62-aa 318）。在48 h干旱处理下，TaSnRK2.1在小麦根、叶中呈明显的干旱诱导表达模式，均在处理24 h后表达量达到峰值，以后至48 h该基因转录维持较高水平。将处理48 h植株转入正常恢复处理后，该基因在根、叶中表达随处理进程不断下调。基因转化分析表明，干旱处理下，与野生型对照（WT）相比，TaSnRK2.1正义表达烟草株系（Sen 1）植株长势增强、干质量增加，气孔关闭加快，脯氨酸、可溶性蛋白、可溶性糖等细胞渗透调节物质含量提高，光合能力和细胞保护酶活性增强；与此相反，该基因反义表达株系（Anti 1）干旱处理下较WT长势减弱、干质量减小，气孔关闭减慢，细胞渗透调节物质含量下降，光合能力和细胞保护酶活性降低。研究表明，TaSnRK2.1通过在转录水平上对干旱胁迫产生应答，参与植株气孔运动、细胞渗透调节、光合和细胞保护过程，增强植株抵御干旱胁迫的能力。 |
| 关 键 词： | 蛋白激酶; 转基因株系; 干旱胁迫 |
| DOI： | 10.57237/j.life.2023.02.002 |
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收稿日期: 2022-12-09
接受日期: 2023-01-30
在线出版日期: 2023-05-25
Functional Characterization on Wheat Protein Kinase Gene TaSnRK2.1 in Mediating Plant Resistance to Drought Stress
College of Agronomy, Hebei Agricultural University, Baoding 071001, China
| Abstract: | The protein kinase type of sucrose non-fermenting-1 related protein kinase 2 (SnRK2) plays an important role in regulating plant growth and development and in mediating plant resistance to abiotic stresses given that it involves transduction of distinct internal and abiotic stress signaling. In this study, TaSnRK2.1, a gene of SnRK2 family in wheat, was investigated focusing on its molecular characterization, expression pattern upon drought stress, and the biological function in mediating plant drought tolerance. At nucleic acid level, TaSnRK2.1 is highly homologous to its counterparts of SnRK2 kinase family genes in species of Polish wheat and barley, whose translated protein harbors a conserved catalytic domain of Ser/Thr kinase domain (aa 62-aa 318). Under a 48 h-regime drought treatment, the transcripts of TaSnRK2.1 showed obvious response in roots and leaves, with the highest expression level reached at 24 h after treatment and maintaining a high level to 48 h under drought condition. Meanwhile, the transcripts of TaSnRK2.1 in both roots and leaves induced by drought were recovered following the normal recovery treatment. Gene transgene analysis indicated that compared with wild type control (WT), the tobacco line with sense-overexpression of TaSnRK2.1 (Sen 1) enlarged plant growth phenotypes, increased dry weights, promoted stomata closing, increased contents of cellular osmtic-regulatory substances including proline, soluble protein, and soluble sugar, enhanced photosynthetic capacity and elevated cellular protective enzyme activities under drought treatment. In contrast, the line with antisense-expression of TaSnRK2.1 (Anti 1) reduced plant growth phenotypes, decreased biomass, slowed stomata closing, decreased the contents of cellular osmtic-regulatory substances, photosynthetic capacity and protective enzyme activities compared with WT plants treated by drought stress. Our investigation suggested that TaSnRK2.1 is sensitive in response to drought signaling at transcriptional level that confers enhanced plant drought resistance by regulating physiological processes associated with stomata movement, cellular osmotic-regulatory substance metabolism, photosynthetsis, and protective enzyme activities. |
| Keywords: | Wheat; SnRK2.1; Gene Expression; Drought Stress; Gene Function |
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