多味中药的抑菌效果研究
西南林业大学, 生命科学学院, 云南昆明 650224
| 摘 要： | 目的：提取枳壳、白头翁、五味子、地榆、黄芩、诃子、大黄、石榴皮、乌梅、黄柏和黄连的有效成分，检测这些中药对产色葡萄球菌、木糖葡萄球菌、伪中间葡萄球菌、雷金斯堡约克氏菌、嗜水气单胞菌和肺炎克雷伯氏菌的体外最小抑菌浓度，为中药在临床治疗中的应用和开发奠定理论基础。方法：采用醇提法提取中药有效成分，利用试管二倍稀释法测定单味中药的最小抑菌浓度。结果：五味子、石榴皮、乌梅和诃子对木糖葡萄球菌有最显著的抑菌效果（MIC均为62.50 mg/mL）；五味子对产色葡萄球菌的抑菌效果最显著（MIC为31.25 mg/mL）；五味子、石榴皮和诃子对伪中间葡萄球菌的抑菌效果最显著（MIC均为31.25 mg/mL）；五味子、地榆、诃子、大黄、乌梅和黄连对雷金斯堡约克氏菌的抑菌效果最显著（MIC均为15.60 mg/mL）；地榆对嗜水气单胞菌的抑菌效果最显著（MIC为1.95 mg/mL）；乌梅对肺炎克雷伯氏菌抑菌效果最显著（MIC为0.98 mg/mL）。结论：五味子、乌梅和诃子等具有较好的抑菌效果，可为这些中药的临床应用提供依据。 |
| 关 键 词： | 中药; 菌株; 最小抑菌浓度; 抑菌效果 |
| DOI： | 10.57237/j.life.2022.01.004 |
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收稿日期: 2022-12-08
接受日期: 2023-03-07
在线出版日期: 2023-03-28
The Study on the Antibacterial Effects of Several Traditional Chinese Medicines
College of Life Sciences, Southwest Forestry University, Kunming 650224, China
| Abstract: | Objective: The effective components of Fructus Aurantii, Pulsatilla chinensis, Schisandra chinensis, Sanguisorba officinalis, Scutellaria baicalensis, Terminalia chebula, Rheum officinale, Punica granatum, Fructus Mume, Cortex Phellodendri and Coptis chinensis were extracted, and the minimum inhibitory concentrations of components from these Traditional Chinese Medicines were analyzed in vitro against Staphylococcus chromogenes, S. xylosus, S. pseudintermedius, Yokenella regensburgei, Aeromonas hydrophila and Klebsiella pneumoniae, so as to lay a theoretical foundation for the application and development of Traditional Chinese Medicine in clinical treatment. Methods: The effective components of Traditional Chinese Medicines were extracted by alcohol extraction, and the minimal inhibitory concentration of single Traditional Chinese Medicine was determined by tube dilution method. Results: S. chinensis, P. granatum, F. Mume and T. chebula showed the most significant bacteriostatic effects on S. xylosus (MICs were all 62.50 mg/mL); S. chinensis presented the most significant antibacterial effect on S. chromogenes (MIC was 31.25 mg/mL); S. chinensis, P. granatum and T. chebula had the most significant bacteriostatic effects on S. pseudointermedia (MICs were all 31.25 mg/mL); S. chinensis, S. officinalis, T. chebula, R. officinale, F. Mume and C. chinensis had the most significant bacteriostatic effects on Y. regensburgei (MICs were all 15.60 mg/mL); The inhibitory effect of S. officinalis on A. hydrophila was the most significant (MIC was 1.95 mg/mL); F. Mume had the most significant antibacterial effect on K. pneumoniae (MIC was 0.98 mg/mL). Conclusion: S. chinensis, F. mume and T. chebula et al. have good antibacterial effects, thus providing basis for the clinical application of these Traditional Chinese Medicines. |
| Keywords: | Traditional Chinese Medicine; Strains; Minimum Inhibitory Concentration; Antibacterial Effects |
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