基于PCR技术检测鸡粪样中的耐药元件
西南林业大学, 生命科学学院, 云南昆明 650224
| 摘 要： | 目的：通过PCR技术从鸡粪样中检测各种耐药元件，以期为PCR技术在肠道菌群耐药性普查的应用上奠定基础。方法：从采集的鸡粪样中提取菌群基因组DNA，采用常规PCR技术扩增不同的耐药元件。结果：本研究从鸡粪样中检测到了不同类的耐药基因tetA、tetB、blaSHV、blaTEM、blaCTX-M1、qnrA、aadA1、dfrA1和sul1，且在饲养的不同节段，不同的耐药基因的检出率存在时间点差异性，其中，tetB随着饲养时间的延长具有逐步增高的趋势，而blaTEM和aadA1随饲养时间延长有下降的趋势。此外，本研究还检测到了整合酶基因intI1和intI2。在质粒复制子类型中，本研究检测出了IncB/O、IncFIC、IncFIA、IncFIB、IncI1、IncN和IncFrep，不同样品携带质粒复制子类型的数量范围为1-6种，且质粒复制子的检出率也存在时间点差异性，但未表现出明显的规律性。在质粒间的关联性方面，检出的质粒间存在不同程度的关联性。结论：采用PCR技术可检测到肠道菌群中的不同耐药元件。 |
| 关 键 词： | PCR技术; 鸡; 粪样; 检测; 耐药元件 |
| DOI： | 10.57237/j.jaf.2023.01.002 |
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收稿日期: 2022-11-30
接受日期: 2023-01-13
在线出版日期: 2023-02-03
Detection of Drug Resistant Elements from Broiler Feces Based on PCR Technology
College of Life Sciences, Southwest Forestry University, Kunming 650224, China
| Abstract: | Objective: The aim of this study is to detect various drug resistance elements from chicken feces by PCR technology, so as to lay a foundation for the application of PCR technology in the survey of drug resistance of intestinal microbiota. Methods: Genomic DNA was extracted from chicken feces, and different drug resistance elements were amplified by conventional PCR. Results: In this study, different classes of the drug-resistant genes tetA, tetB, blaSHV, blaTEM, blaCTX-M1, qnrA, aadA1, dfrA1 and sul1 were observed from collected chicken feces, and the detection rates of different drug-resistant genes varied based on time point at which the genes were amplified. Wherein, tetB progressively increased with the prolongation of feeding time, while blaTEM and aadA1 decreased with the prolongation of feeding time. In addition, integrase genes intI1 and intI2 were also detected in this study. Among the types of plasmid replicons, IncB/O, IncFIC, IncFIA, IncFIB, IncI1, IncN and IncFrep were detected in this study, and the number of replicon types carried by different samples ranged from 1 to 6, and the detection rate of plasmid replicons also had a time point difference, while, obvious regularity was not observed. In terms of the correlations between plasmids, the detected plasmids presented varying degrees of correlations. Conclusion: PCR technology could detect different drug resistance elements in intestinal microbiota. |
| Keywords: | PCR Technology; Broiler; Fece Samples; Detection; Drug Resistant Elements |
基金
| 1. | 云南省教育厅科学研究基金项目 (2022J0505). |
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