Effects of Astragalus Polysaccharides on iNOS-NO Induced by Lipopolysaccharide in Human Thyroid Cells
1. The Department of Geriatrics, The First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121001, China
2. The Department of Pharmacology, Jinzhou Medical University, Jinzhou 121001, China
| Abstract: | Objective: To study the effects of Astragalus polysaccharides on the induction of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide (LPS) in human thyroid cells in vitro. Methods: Thyrocytes obtained from paraadenomatous tissue of thyroid adenomas were cultured in a monolayer system. Human thyrocytes were treated with LPS at different concentration (0, 1, 10, 100μg • ml-1) and at the same concentration for different time (6, 12, 18, 24 h). The level of nitric oxide (NO) in the culture medium was observed; Effect of iNOS inhibitor L-NAME on the above-mentioned effects of LPS; Effect of Astragalus polysaccharides on the above-mentioned effects of LPS. Results: (1) LPS (0, 1, 10, 100μg•ml-1) significantly increased the level of NO in dose-dependent manner. (2) LPS (6, 12, 18, 24 h) significantly increased the level of NO in time-dependent manner at the same concentration. (3) L-NAME inhibited the above-mentioned effects of LPS. (4) Astragalus polysaccharides inhibited the above-mentioned effects of LPS. Conclusions: Astragalus polysaccharides inhibited LPS-induced NO synthesis in iNOS in thyroid cells. Astragalus polysaccharides might have therapeutic effects on thyroid-related diseases caused by LPS-mediated infection. |
| Keywords: | Astragalus Polysaccharides; Lipopolysaccharide; Inducible Nitric Oxide Synthase; Nitric Oxide; Thyroid |
| DOI: | 10.57237/j.wjcm.2023.03.001 |
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Received: 31 May 2023
Accepted: 14 July 2023
Published Online: 24 July 2023
Funding
| 1. | 辽宁省锦州市指导性科技计划项目 (JZ2023B057) |
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