Genetic Analysis of Megalobrama amblycephala, Ancherythroculter nigrocauda and Bo Fang “Xianfeng No. 2” Based on SNP Markers
Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430207, China
| Abstract: | The SNP markers of Megalobrama amblycephala, Ancherythroculter nigrocauda and Bo Fang “Xianfeng No. 2” were developed by SLAF-seq in this study. Further analysis on genetic diversity and genetic population were also done in this paper. The results showed that the efficiency of Enzyme digestion was 90.11% and 181.13M reads were obtained from three fishes. 1,339,007 SLAF labels and 446,680 SNP markers were through bioinformatics analysis. And the Q30 of mean sequencing was 93.03%, the mean GC content was 37.79%. The results showed that the observed allele number (No) was 2.0, and the expected allele number (Ne) was 1.63. The observed heterozygosity (Ho) was 0.08, while the expected heterozygosity (He) was 0.36. The genetic diversity index was 0.41, the Shnnon Wiener index was 0.54, and the PIC was 0.29, the MAF was 0.28. These results suggest that the genetic diversity of three populations were in the medium level. It might be related to artificial selection. The nearest genetic distance was between Bo Fang “Xianfeng No. 2” and Megalobrama amblycephala, and the furthest distance between was Megalobrama amblycephala and Ancherythroculter nigrocauda, which were in accordance with their shapes. |
| Keywords: | Megalobrama amblycephala; Ancherythroculter nigrocauda; Bo Fang “Xianfeng No. 2”; SNP Marker; SLAF-seq; Genetic Diversity; Genetic Population |
| DOI: | 10.57237/j.jaf.2023.03.003 |
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Received: 10 August 2023
Accepted: 20 September 2023
Published Online: 9 October 2023
Funding
| 1. | 湖北省科技创新专项 (2018ABA105) |
| 2. | 湖北洪山实验室开放课题 (2021hskf015) |
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