Detection of Drug Resistant Elements from Broiler Feces Based on PCR Technology
College of Life Sciences, Southwest Forestry University, Kunming 650224, China
| Abstract: | Objective: The aim of this study is to detect various drug resistance elements from chicken feces by PCR technology, so as to lay a foundation for the application of PCR technology in the survey of drug resistance of intestinal microbiota. Methods: Genomic DNA was extracted from chicken feces, and different drug resistance elements were amplified by conventional PCR. Results: In this study, different classes of the drug-resistant genes tetA, tetB, blaSHV, blaTEM, blaCTX-M1, qnrA, aadA1, dfrA1 and sul1 were observed from collected chicken feces, and the detection rates of different drug-resistant genes varied based on time point at which the genes were amplified. Wherein, tetB progressively increased with the prolongation of feeding time, while blaTEM and aadA1 decreased with the prolongation of feeding time. In addition, integrase genes intI1 and intI2 were also detected in this study. Among the types of plasmid replicons, IncB/O, IncFIC, IncFIA, IncFIB, IncI1, IncN and IncFrep were detected in this study, and the number of replicon types carried by different samples ranged from 1 to 6, and the detection rate of plasmid replicons also had a time point difference, while, obvious regularity was not observed. In terms of the correlations between plasmids, the detected plasmids presented varying degrees of correlations. Conclusion: PCR technology could detect different drug resistance elements in intestinal microbiota. |
| Keywords: | PCR Technology; Broiler; Fece Samples; Detection; Drug Resistant Elements |
| DOI: | 10.57237/j.jaf.2023.01.002 |
View:
Downloads:
Received: 30 November 2022
Accepted: 13 January 2023
Published Online: 3 February 2023
Funding
| 1. | 云南省教育厅科学研究基金项目 (2022J0505). |
References
| [1] | 周芷锦, 陈洁, 沈红霞,等. 动物源细菌耐药性评估在兽用抗菌药使用减量化行动中的应用 [J]. 中国兽药杂志, 2022, 56 (7): 6-11. |
| [2] | Martínez JL, Coque TM, Baquero F. What is a resistance gene? Ranking risk in resistomes [J]. Nat Rev Microbiol. 2015, 13 (2): 116-123. |
| [3] | Hu Y, Yang X, Li J, et al. The Bacterial Mobile Resistome Transfer Network Connecting the Animal and Human Microbiomes [J]. Appl Environ Microbiol. 2016, 82 (22): 6672-6681. |
| [4] | Swift BMC, Bennett M, Waller K, et al. Anthropogenic environmental drivers of antimicrobial resistance in wildlife [J]. Sci Total Environ. 2019, 649: 12-20. |
| [5] | 陆东东,张伟. 多药耐药基因检测方法介绍 [J]. 陕西医学检验,2000, 15 (3): 8. |
| [6] | Chen YM, Holmes EC, Chen X, et al. Diverse and abundant resistome in terrestrial and aquatic vertebrates revealed by transcriptional analysis [J]. Scientific Reports, 2020, 10 (1): 18870. |
| [7] | Forsberg KJ, Reyes A, Wang B, et al. The Shared Antibiotic Resistome of Soil Bacteria and Human Pathogens [J]. Science, 2012, 337 (6098): 1107-1111. |
| [8] | Bich V, Le VT, Thai PD, et al. An exploration of the gut and environmental resistome in a community in northern Vietnam in relation to antibiotic use [J]. Antimicrobial Resistance and Infection Control, 2019, 8: 194. |
| [9] | 王晓泉, 王彦红, 吴双, 等. 四环素耐药基因在鸡源沙门菌中的分布和传播 [J]. 中国家禽, 2007, 29 (9): 10-12. |
| [10] | 李胜利. 河南省鸭源大肠杆菌四环素类及磺胺类耐药基因的分子检测 [J]. 黑龙江畜牧兽医, 2016, 11: 174-176. |
| [11] | 杨承霖, 舒刚, 赵小玲, 等. 2010-2016年四川省食品动物源大肠杆菌的耐药性研究 [J]. 西北农林科技大学学报（自然科学版）, 2020, 48 (9): 8. |
| [12] | 钟嘉诚, 仇桂玲, 陈济铛, 等. 广东地区禽源致病性大肠杆菌对5种β-内酰胺类抗生素的耐药情况与ESBLs基因的携带情况 [J]. 黑龙江畜牧兽医, 2021, 16: 62-72. |
| [13] | 郭家媚, 葛成, 张文斌, 等. 水貂源致病性大肠杆菌对喹诺酮类药物耐药表型与耐药基因型检测与分析 [J]. 野生动物学报, 2021, 42 (1): 166-170. |
| [14] | 顾晓晓, 邬琴, 陶乔孝慈, 等. 羊源大肠杆菌对氨基糖苷类药物耐药表型及耐药基因的检测 [J]. 中国兽医学报, 2020, 40 (8): 1566-1570. |
| [15] | 杨瑞, 党儒尧, 何云凤, 等. 2019年-2020年河北地区鸡源沙门菌流行病学调查及耐药性分析 [J]. 中国预防兽医学报, 2021, 43 (9): 924-929. |
| [16] | 孙宇洁, 孟令轩, 朱琳, 等. 水产养殖尾水中抗生素抗性基因的去除及磺胺类耐药细菌研究 [J]. 环境科学学报, 2022, 42 (5): 286-295. |
| [17] | 闫爽, 冯涛, 王东阳, 等. 东北地区狐源大肠杆菌耐药性,整合酶及整合子基因的检测与相关分析 [J]. 中国预防兽医学报, 2022, 44 (4): 439-444. |
| [18] | 鲁勇, 汪一萍, 应建飞, 等. 临床分离肠杆菌科细菌Ⅰ型整合子和ISCR1的检测 [J]. 中国卫生检验杂志, 2021, 31 (15): 1832-1834. |
| [19] | 苟秋凤, 代富英, 曹康, 等. 全基因组测序后质粒的组装与鉴定研究进展 [J]. 成都医学院学报, 2022, 17 (4): 525-529. |
Join the Editorial Board
We invite active, qualified and high profile scientists and researchers to join as Editorial Board Members.
Join UsBecome a Reviewer
Scholars with a strong interest in reviewing are invited to join the reviewer panel to ensure the quality of the research to be published.
Join Us